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The male partner is important in the assessment of the infertile couple. Here at Pacific Northwest
Fertility and IVF Specialists, we offer a full range of semen testing as well as the preparation of semen
for insemination. Below are the tests and preparations methods we offer at our Laboratory.
Seminal Fluid Analysis (SA)
A seminal fluid analysis is one of the first diagnostic assays in the assessment of male
fertility potential. It includes determination of sperm concentration (millions/ml), percent motility,
speed and forward progression of motile sperm, morphological analysis of normal vs abnormal sperm
(Krueger Strict) or percent of morphologically normal sperm including identification and percent of
abnormal forms (WHO or World Health Organization). White blood cells and non-tailed forms (immature
sperm) are identified with differential staining, and seminal pH, presence or absence of
agglutination, and viscosity are noted.
Sperm Penetration Assay (SPA)
The sperm penetration assay can assess the
ability of the male to potentially penetrate and
activate an egg. The patient’s sperm is exposed to
treated hamster eggs after a period of cold
capacitation, and the capacitation index or average
number of sperm penetrating each egg is reported. It
can be an additional test when the seminal fluid
analysis is normal.
Immunobead Test (IMB)
This assay is performed on blood sera, seminal
fluids, or sperm and may detect sperm directed
antibodies the could interfere with sperm transport
or sperm-egg fusion. IgA and IgG antibodies are
measured. IgM antibodies are not present in the
reproductive tract. This test is useful in cases of
idiopathic infertility, as well as vasecectomy
reversal or trauma/surgery in the groin area in the
male partner.
Fructose Assay (FS)
This is a test for the presence of fructose in
the semen. Fructose is produced by the seminal
vesicles and normally found in semen. The test is
performed on azoospermic patients with no medical
history that would indicate the absence of sperm ie:
prior chemotherapy or vasectomy. Absence of fructose
may indicate obstruction of an ejaculatory duct or
absence of the seminal vesicles.
Sperm Separation For Intrauterine Insemination (Density Gradient)
This procedure isolates motile sperm from
immotile sperm and other components in the seminal
plasma , including most bacteria and white blood
cells. It also may be used to prepare retrograde
ejaculated sperm for intrauterine insemination.
Sperm Cryopreservation (SC)
Sperm cryopreservation (freezing) may be done for
several reasons: cancer patients preparing to
undergo radiation or chemotherapy that potentially
may destroy sperm production, patients who are
electing to have a vasectomy but wish to have sperm
stored, and patients who are unavailable and wish to
store sperm for their partner’s use.
Cryostorage of Anonymous Donor Sperm
Donor sperm cryopreserved at another facility may
be brought in and stored at Pacific North West
Fertility with appropriate documentation of testing
and consent to storage signed by the patient. Known
Donor sperm may also be frozen and stored after
appropriate disease testing, consents, and
quarantine.
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